A simplified AFLP method for fingerprinting of common wheat (Triticum aestivum L.) cultivars.

Dna Fingerprinting Studies of Some Wheat (triticum Aestivum L.) Genotypes Using Random Amplified Polymorphic Dna (rapd) Analysis

Random amplified polymorphic DNA (RAPD) analysis was employed to estimate genetic diversity/genetic similarity among nine wheat (Triticum aestivum L.) genotypes. Out of 25 random 10-mer primers surveyed, 18 primers detected polymorphism among all the wheat genotypes, while 7 primers produced monomorphic pattern. A total of 175 bands were amplified, 60.57% of which were polymorphic. Genetic simi...

Fingerprinting and identification of closely related wheat (Triticum aestivum L.) cultivars using ISSR and fluorescence-labeled TP-M13-SSR markers

DNA based molecular markers allow precise, objective and rapid cultivar identification. In this study, five ISSR and two sets of fluorescence-labeled TP-M13-SSR markers were applied for discrimination of eight closely related wheat cultivars. Five ISSR primers revealed a total of 43 distinct reproducible bands, 29 (67.44%) of the 43 bands were polymorphic. The number of polymorphic bands detect...

Chromosomal location of AFLP markers in common wheat utilizing nulli-tetrasomic stocks.

Amplified fragment length polymorphism (AFLP) markers with a total of 256 EcoRI + ANN - MseI + CNN primer combinations were investigated employing the common wheat cultivar Triticum aestivum 'Chinese Spring.' On average, 103 fragments per primer combination were amplified, ranging from a maximum of 226 fragments to a minimum of 18 fragments. The primer combinations E + AAA - M + CNN and E + ATT...

Effect of soil moisture stress on root and shoot development of seven wheat (triticum aestivum L.) cultivars

High density mapping and haplotype analysis of the major stem-solidness locus SSt1 in durum and common wheat

Breeding for solid-stemmed durum (Triticum turgidum L. var durum) and common wheat (Triticum aestivum L.) cultivars is one strategy to minimize yield losses caused by the wheat stem sawfly (Cephus cinctus Norton). Major stem-solidness QTL have been localized to the long arm of chromosome 3B in both wheat species, but it is unclear if these QTL span a common genetic interval. In this study, we h...